Cytochalasin C is primarily used in biological research to study cellular processes, particularly those involving the cytoskeleton. It inhibits actin polymerization, which makes it a valuable tool for investigating cell motility, shape changes, and intracellular transport. Researchers utilize it to disrupt actin filaments, allowing them to observe the effects on cell division, migration, and signaling pathways. Additionally, it has been employed in studies of endocytosis and exocytosis, as actin dynamics play a critical role in these processes. Its ability to interfere with actin networks also makes it useful in cancer research, where it helps explore the role of the cytoskeleton in tumor cell invasion and metastasis.