Pentafluorophenyl benzoate is primarily utilized in organic synthesis as a versatile reagent, particularly in peptide coupling reactions. Its strong electron-withdrawing pentafluorophenyl group enhances the reactivity of the ester, making it an effective acylating agent. This compound is often employed in the preparation of amides, esters, and other derivatives, especially in cases where mild reaction conditions are required. Additionally, it serves as a building block in the development of advanced materials and pharmaceuticals, where precise functionalization is critical. Its stability and reactivity also make it suitable for use in solid-phase synthesis and combinatorial chemistry.