DL-beta-Hydroxybutyryl coenzyme A lithium salt is primarily used in biochemical research to study metabolic pathways, particularly those involving ketone bodies and fatty acid metabolism. It serves as a substrate or intermediate in enzymatic assays to investigate the activity of enzymes like beta-hydroxybutyryl-CoA dehydrogenase. This compound is also utilized in studies related to energy metabolism, especially in conditions such as ketosis or starvation, where the body relies on ketone bodies for energy. Additionally, it aids in understanding disorders of lipid metabolism and mitochondrial function, providing insights into diseases like diabetes and obesity. Its application extends to the development of therapeutic strategies targeting metabolic dysregulation.