Acylase from Aspergillus sp. is widely used in the pharmaceutical industry for the production of optically pure amino acids, which are crucial intermediates in the synthesis of various drugs. It plays a key role in the resolution of racemic mixtures, enabling the separation of enantiomers to produce single-isomer compounds with higher therapeutic efficacy. This enzyme is also employed in the food industry to enhance flavor profiles by modifying amino acid derivatives. Additionally, it is utilized in biotechnology for the synthesis of chiral compounds and in the development of biosensors for detecting specific amino acids. Its application in environmental biotechnology includes the degradation of acylated pollutants, contributing to bioremediation efforts.