Amidase from Pseudomonas aeruginosa is widely used in biocatalysis and industrial processes due to its ability to hydrolyze amide bonds, making it valuable for the production of carboxylic acids and amines. It is applied in the synthesis of pharmaceuticals, where it aids in the resolution of racemic mixtures to produce enantiomerically pure compounds, essential for drug development. Additionally, this enzyme is utilized in environmental biotechnology for the degradation of amide-containing pollutants, contributing to wastewater treatment and soil remediation. Its role in hydroxamate transferase activity further extends its application in the synthesis of hydroxamic acids, which are important in medicinal chemistry and as metal chelators. The recombinant expression in E. coli ensures high yield and purity, making it a reliable tool for research and industrial applications.