Penicillin amidase from Escherichia coli is widely used in the pharmaceutical industry for the production of semi-synthetic antibiotics. It plays a crucial role in the enzymatic hydrolysis of penicillin G to produce 6-aminopenicillanic acid (6-APA), a key intermediate in the synthesis of various penicillin derivatives. This enzyme is also employed in the synthesis of cephalosporins, another important class of antibiotics. Its specificity and efficiency make it valuable in biotransformation processes, enabling the production of high-purity pharmaceutical compounds. Additionally, it is utilized in research and development for studying enzyme kinetics and protein engineering to enhance its catalytic properties for industrial applications.