CI-949 is primarily utilized in research settings as a selective agonist for the adenosine A1 receptor. Its application is significant in studying the physiological and pathological roles of adenosine receptors in various tissues. Researchers employ CI-949 to investigate its effects on cardiac function, particularly in modulating heart rate and reducing myocardial oxygen demand, which is crucial for understanding heart-related conditions. Additionally, it is used in neuroscience research to explore its potential neuroprotective properties and its role in modulating neurotransmitter release. The compound's ability to selectively activate the A1 receptor makes it a valuable tool in pharmacological studies aimed at developing targeted therapies for cardiovascular and neurological disorders.