Akt Inhibitor IV is primarily used in biochemical research to study cellular signaling pathways, particularly the PI3K/Akt/mTOR pathway, which plays a critical role in cell survival, growth, and metabolism. It is employed to inhibit Akt activity, allowing researchers to investigate the effects of Akt suppression on cancer cell proliferation, apoptosis, and resistance to chemotherapy. This compound is also utilized in studies exploring metabolic disorders, as Akt signaling is involved in insulin regulation and glucose metabolism. Additionally, it aids in understanding the role of Akt in neurodegenerative diseases and cardiovascular conditions, providing insights into potential therapeutic targets. Its application extends to drug discovery, where it helps in identifying and validating new compounds targeting the Akt pathway for treating various diseases.