PF-AKT400 is primarily utilized in biochemical research as a potent and selective inhibitor of the AKT signaling pathway. It is employed in studies aimed at understanding the role of AKT in cellular processes such as growth, survival, and metabolism. Researchers use PF-AKT400 to investigate the effects of AKT inhibition in various cancer models, as AKT is often hyperactivated in many types of tumors. By blocking AKT activity, this compound helps in elucidating potential therapeutic strategies for cancer treatment. Additionally, PF-AKT400 is used in preclinical studies to assess its efficacy and safety profile, contributing to the development of targeted therapies for diseases driven by aberrant AKT signaling.