Pentacontane is primarily utilized in research and industrial applications due to its long hydrocarbon chain. It serves as a standard in chromatography for calibrating instruments and analyzing complex hydrocarbon mixtures. In material science, it is used to study the properties of long-chain alkanes, particularly their phase behavior and crystallization processes. Additionally, pentacontane is employed in the development of lubricants and coatings, where its stability and hydrophobic nature are advantageous. It also finds use in the synthesis of specialized polymers and as a reference compound in the study of waxes and paraffin-like materials.