4-hydroperoxy Cyclophosphamide is primarily used in research settings to study the mechanisms of chemotherapy and drug resistance. It serves as an active metabolite of cyclophosphamide, allowing scientists to investigate its cytotoxic effects on cancer cells without requiring metabolic activation. This compound is particularly valuable in in vitro studies, where it helps elucidate the pathways involved in DNA damage and apoptosis in tumor cells. Additionally, it is utilized to explore the development of resistance mechanisms in cancer cells, aiding in the design of more effective therapeutic strategies. Its role in immunology research is also notable, as it helps in understanding its immunosuppressive effects and potential applications in autoimmune disease studies.