Used in bioconjugation and chemical biology research, this compound serves as a versatile linker for attaching biomolecules, such as proteins or peptides, to other entities like fluorescent dyes or solid supports. Its biotin moiety enables strong binding to streptavidin or avidin, facilitating purification or detection in assays. The PEG4 spacer enhances solubility and reduces steric hindrance, while the alkyne group allows for click chemistry reactions, such as copper-catalyzed azide-alkyne cycloaddition, for site-specific labeling. The tert-butyl group provides stability, making it suitable for complex synthetic routes. This compound is particularly valuable in drug discovery, proteomics, and the development of targeted therapeutics, where precise molecular interactions are critical.