This compound is widely used in bioconjugation and click chemistry applications due to its reactive NHS ester and DBCO (dibenzocyclooctyne) groups. The NHS ester allows for efficient coupling with primary amines, making it ideal for labeling proteins, peptides, and other amine-containing biomolecules. The DBCO group enables bioorthogonal reactions with azide-functionalized molecules, facilitating rapid and selective conjugation without the need for catalysts. This makes it valuable in drug delivery systems, antibody-drug conjugates, and the development of diagnostic probes. Its PEG4 spacer enhances solubility and reduces steric hindrance, improving reaction efficiency in aqueous environments.