DBCO-PEG4-DBCO is widely used in bioconjugation and click chemistry applications due to its dual DBCO (dibenzocyclooctyne) groups, which enable efficient and selective reactions with azide-containing molecules. Its PEG4 spacer provides flexibility and solubility, making it ideal for linking biomolecules without compromising their functionality. This compound is particularly valuable in the development of antibody-drug conjugates (ADCs), where it facilitates the precise attachment of cytotoxic drugs to antibodies, enhancing targeted cancer therapy. Additionally, it is employed in the labeling and imaging of biomolecules, enabling researchers to track cellular processes and molecular interactions with high specificity. DBCO-PEG4-DBCO is also utilized in surface modification and material science, where it aids in creating functionalized surfaces for biosensors, drug delivery systems, and tissue engineering scaffolds. Its versatility and reliability make it a key tool in advancing biomedical research and therapeutic innovations.