L-Homopropargylglycine hydrochloride is widely used in biochemical research, particularly in the study of protein synthesis and labeling. It serves as a metabolic precursor for incorporating alkyne tags into proteins, enabling their visualization and tracking through bioorthogonal chemistry techniques like click chemistry. This compound is valuable in proteomics for identifying and analyzing newly synthesized proteins in cells. It is also employed in the development of novel therapeutics and diagnostics, aiding in the understanding of cellular processes and disease mechanisms. Additionally, it finds application in chemical biology for studying post-translational modifications and protein-protein interactions.