Diazo Biotin-Alkyne is widely used in chemical biology and proteomics for labeling and detecting biomolecules. It is particularly valuable in bioorthogonal chemistry, where its alkyne group enables selective reactions with azide-containing compounds through click chemistry. This property allows researchers to tag and visualize specific proteins, nucleic acids, or other biomolecules in complex biological systems. Additionally, the biotin moiety facilitates affinity purification or detection using streptavidin-based methods, making it a versatile tool for studying protein interactions, post-translational modifications, and cellular processes. Its diazo group can also be utilized for photoaffinity labeling, enabling the capture of transient molecular interactions in live cells. Overall, it is a powerful reagent for advanced biochemical research and diagnostics.