Used in bioorthogonal chemistry for site-specific bioconjugation, particularly in labeling biomolecules such as peptides, proteins, and antibodies. Its strained alkyne structure enables fast and selective reactions with azides without interfering with biological processes. The pentanoic acid functional group allows for easy coupling to amines via standard EDC/NHS chemistry, making it useful in developing antibody-drug conjugates, imaging probes, and targeted therapeutics. Also employed in materials science for surface modification and functionalization of polymers and nanoparticles.