Used in bioorthogonal chemistry for live-cell imaging, enabling real-time tracking of biomolecules without interfering with natural biological processes. Its near-infrared fluorescence allows deep tissue imaging with low background signal, making it ideal for in vivo studies. Commonly employed in click chemistry reactions, especially strain-promoted azide-alkyne cycloaddition (SPAAC), to label azide-modified biomolecules in complex biological environments. Suitable for fluorescence microscopy, flow cytometry, and molecular imaging applications where high sensitivity and minimal photodamage are required.