Used primarily in biochemical research, this compound acts as a nucleoside analog, enabling the study of RNA synthesis and metabolism. It is particularly valuable in click chemistry applications, where its alkyne group facilitates selective labeling and detection of RNA molecules in live cells. This allows researchers to track RNA dynamics and investigate gene expression patterns with high precision. Additionally, it serves as a tool in the development of antiviral and anticancer therapies by targeting RNA-dependent processes. Its incorporation into RNA strands helps in understanding structural and functional aspects of RNA, aiding in the design of therapeutic interventions.