This chemical is widely used in bioconjugation and click chemistry applications due to its dibenzocyclooctyne (DBCO) group, which reacts efficiently with azides without the need for a catalyst. Its dual polyethylene glycol (PEG2) linkers enhance solubility and biocompatibility, making it suitable for use in biological systems. The bis design with two carboxylic acid-terminated arms allows for multifunctional conjugation to amine-containing molecules. It is commonly employed in the labeling and modification of biomolecules such as proteins, antibodies, and nucleic acids for research, diagnostics, and therapeutic purposes. Additionally, it is utilized in the development of drug delivery systems, where its ability to form stable linkages is crucial for targeting and controlled release mechanisms, as well as in nanotechnology applications like surface modification of nanoparticles, viruses, or cells.