This chemical is primarily utilized in bioconjugation and click chemistry applications. Its structure, featuring DBCO (dibenzocyclooctyne) and NHS ester groups, makes it highly effective for labeling biomolecules such as proteins, peptides, and antibodies. The DBCO group enables strain-promoted alkyne-azide cycloaddition (SPAAC), a copper-free click chemistry reaction, which is particularly useful in sensitive biological systems where copper toxicity is a concern. The NHS ester groups allow for efficient covalent attachment to primary amines, facilitating the modification of biomolecules. This compound is widely used in drug delivery systems, diagnostic assays, and the development of targeted therapies, where precise and stable conjugation is essential. Its PEG (polyethylene glycol) spacer enhances solubility and reduces steric hindrance, improving the efficiency of the conjugation process.