This compound is widely used in bioconjugation applications, particularly in click chemistry reactions. Its DBCO (dibenzocyclooctyne) group allows for efficient, catalyst-free reactions with azide-containing molecules, making it valuable for labeling and modifying biomolecules such as proteins, antibodies, and nucleic acids. The NHS ester group enables covalent bonding with primary amines, facilitating the attachment of the compound to target molecules. It is commonly employed in drug delivery systems, diagnostic assays, and the development of bioconjugates for research and therapeutic purposes. Its PEG (polyethylene glycol) spacer enhances solubility and reduces steric hindrance, improving reaction efficiency.