Used in chemical biology and medicinal chemistry as a versatile building block for click chemistry reactions, particularly in the synthesis of bioconjugates and probes. Its alkyne group enables efficient copper-catalyzed azide-alkyne cycloaddition (CuAAC), making it valuable for labeling biomolecules like proteins, nucleic acids, and carbohydrates. The ester functional group allows for further modifications or hydrolysis to carboxylic acids, enhancing its utility in drug design and development. Additionally, it serves as a key intermediate in the preparation of complex molecules for biological studies, including enzyme inhibitors and fluorescent tags for imaging applications.