2-Octynoic acid is primarily used in organic synthesis as a building block for more complex chemical compounds. It serves as an intermediate in the production of pharmaceuticals, agrochemicals, and specialty chemicals. Its alkyne functional group makes it valuable in click chemistry reactions, enabling the creation of diverse molecular structures. Additionally, it is utilized in the development of fragrances and flavors due to its ability to contribute unique aromatic properties. In research, it is employed in the study of reaction mechanisms and the synthesis of novel organic molecules.