N'-Hydroxyacetimidamide is primarily utilized in organic synthesis as a versatile intermediate for the preparation of various heterocyclic compounds. It plays a significant role in the development of pharmaceuticals, particularly in the synthesis of oxadiazoles and other nitrogen-containing heterocycles, which are often explored for their biological activities. Additionally, it is employed in the creation of chelating agents and ligands for metal coordination chemistry, aiding in the study of catalytic processes. Its reactive functional groups make it valuable in the modification of polymers and materials, enhancing their properties for specific industrial applications.