Used as a fluorescent derivatization reagent for the sensitive detection of primary and secondary amines in biological samples, particularly in high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). It enables the analysis of amino acids (including those without natural fluorescence such as proline and hydroxyproline), peptides, and neurotransmitters at very low concentrations due to its strong fluorescence signal upon reaction with amine groups. Commonly applied in neurochemical research, metabolic profiling, medical diagnostics, and food quality control where trace-level amine detection is critical.