Used in biochemical and medical research to quantitatively measure valine levels in biological samples such as serum, plasma, or cell culture supernatants. The competition-based ELISA format allows for high sensitivity and specificity, making it suitable for studying amino acid metabolism, nutritional status, and metabolic disorders like maple syrup urine disease. It is also applied in pharmaceutical research to monitor valine concentration in drug development and metabolic studies. The kit enables high-throughput analysis in clinical diagnostics and laboratory research settings.