L-Alanine isopropyl ester hydrochloride is primarily used in organic synthesis as a building block for peptides and other complex molecules. It serves as a protected form of L-alanine, allowing controlled reactions in peptide bond formation. This compound is also utilized in pharmaceutical research for developing drug candidates, particularly in the synthesis of amino acid derivatives. Additionally, it finds application in biochemical studies for investigating enzyme mechanisms and protein interactions due to its stability and reactivity. Its ester group enhances solubility in organic solvents, making it suitable for various chemical processes.