Used in peptide synthesis, particularly in the preparation of osteocalcin and other bone-related proteins containing gamma-carboxyglutamic acid (Gla) residues. The Z and tert-butyl protecting groups allow selective deprotection and controlled formation of complex peptides. Commonly applied in research on blood coagulation and bone metabolism due to its role in synthesizing biologically active Gla-containing peptides. Stable under standard coupling conditions and compatible with solid-phase peptide synthesis methods.