Pyridoxylidene-L-glutamic acid dipotassium salt is primarily utilized in the field of biochemistry and medical research. It serves as a key component in the study of enzymatic reactions, particularly those involving vitamin B6 derivatives. This compound is often employed in the development of diagnostic assays to measure enzymatic activity in biological samples, aiding in the detection of metabolic disorders. Additionally, it is used in the formulation of nutritional supplements and therapeutic agents designed to address deficiencies in vitamin B6, supporting neurological health and metabolic functions. Its stability and solubility make it suitable for use in various pharmaceutical preparations and research applications.