DL-Thyronine is primarily used in biochemical research to study thyroid hormone activity and metabolism. It serves as a reference compound for investigating the effects of thyroid hormones on various physiological processes, including metabolism, growth, and development. Researchers utilize it to explore the mechanisms of thyroid hormone receptors and their role in gene expression. Additionally, DL-Thyronine is employed in the development of assays to measure thyroid hormone levels and to evaluate the efficacy of thyroid-related pharmaceuticals. Its application extends to studying metabolic disorders and potential therapeutic interventions for thyroid dysfunction.