Potassium β-hydroxypyruvate is primarily utilized in biochemical research as a key intermediate in metabolic studies. It plays a significant role in understanding the glycolysis and gluconeogenesis pathways, particularly in the conversion of serine to pyruvate. This compound is also employed in enzymatic assays to study the activity of enzymes like hydroxypyruvate reductase, which is crucial in plant metabolism. Additionally, it finds application in the synthesis of various organic compounds and pharmaceuticals, serving as a building block in the production of bioactive molecules. Its role in metabolic engineering and biotechnological processes further highlights its importance in advancing research related to cellular energy metabolism and biosynthesis pathways.