N’-Formylkynurenine plays a significant role in biochemical research, particularly in the study of tryptophan metabolism. It is an intermediate in the kynurenine pathway, which is crucial for understanding various physiological and pathological processes. Researchers utilize it to investigate the mechanisms of immune regulation, as the kynurenine pathway is closely linked to immune system modulation and inflammation. Additionally, it is studied in the context of neurodegenerative diseases, such as Alzheimer’s and Parkinson’s, where dysregulation of this pathway is often observed. Its role in cancer research is also notable, as altered tryptophan metabolism is associated with tumor immune evasion. Furthermore, it serves as a biomarker in clinical studies to assess oxidative stress and inflammatory conditions. Its applications extend to drug development, where targeting the kynurenine pathway holds potential for therapeutic interventions in various diseases.