3-Fluoro-L-tyrosine hydrochloride is primarily used in biochemical research as a labeled amino acid. It serves as a tool in studying protein synthesis, enzyme mechanisms, and metabolic pathways. The fluorine atom in its structure allows for tracking and imaging using techniques like nuclear magnetic resonance (NMR) spectroscopy and positron emission tomography (PET). This compound is also employed in investigating the role of tyrosine in cellular processes, including signal transduction and neurotransmitter production. Additionally, it aids in the development of novel pharmaceuticals targeting tyrosine-related pathways in diseases such as cancer and neurological disorders. Its application extends to understanding protein folding and stability, providing insights into misfolding diseases like Alzheimer's and Parkinson's.