Isepamicin sulphate is primarily used as an antibiotic in the treatment of severe bacterial infections. It is effective against a wide range of Gram-negative and some Gram-positive bacteria, making it valuable in combating infections in the respiratory tract, urinary tract, and abdominal region. It is often administered in hospital settings for patients with complicated infections, including those caused by multidrug-resistant organisms. Additionally, it is used in cases where other aminoglycoside antibiotics have failed due to resistance. Its application is particularly critical in treating infections in immunocompromised patients or those with serious underlying health conditions. Proper dosage and monitoring are essential to minimize potential side effects, such as nephrotoxicity and ototoxicity.