GYKI53655 hydrochloride is primarily utilized in neuroscience research as a selective and potent antagonist of AMPA receptors. Its application is crucial in studying the role of AMPA receptors in various neurological processes, including synaptic plasticity, learning, and memory. Researchers employ this compound to investigate the mechanisms underlying excitatory neurotransmission and its implications in neurological disorders such as epilepsy, stroke, and neurodegenerative diseases. Additionally, GYKI53655 hydrochloride is used in experimental models to explore potential therapeutic strategies targeting AMPA receptor dysfunction, providing insights into drug development for conditions associated with excessive excitatory signaling in the brain.