Used primarily in microbiology for the cultivation of anaerobic bacteria, especially fastidious anaerobes such as Bacteroides and Fusobacterium species. It supports the growth of organisms that require a rich, reducing environment with specific nutrients and growth factors. Commonly used in clinical laboratories for isolating anaerobic pathogens from clinical specimens like blood, abscesses, and tissue samples. Its formulation promotes the recovery of anaerobes while inhibiting the growth of facultative anaerobes and aerobes when used in anaerobic chambers or jars. Also employed in research settings to study anaerobic bacterial physiology and antibiotic susceptibility.