N-Acetyl-o-phenetidine is primarily used in the synthesis of pharmaceuticals, particularly in the production of analgesics and antipyretics. It serves as an intermediate in the manufacturing of compounds that help reduce pain and fever. Additionally, it is utilized in research and development for creating new therapeutic agents, especially in the field of medicinal chemistry. Its role in organic synthesis also extends to the preparation of various chemical derivatives that may have potential applications in drug discovery and development.