HEPES sodium salt (HEPES-Na)
≥99.5% (titration)
science Other reagents with same CAS 75277-39-3
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description Product Description
HEPES sodium salt is widely used as a buffering agent in biological and biochemical research. It is particularly valued for its ability to maintain a stable pH in cell culture media, enzyme assays, and protein purification processes. The buffer is effective in the physiological pH range of 7.2 to 8.2, making it suitable for maintaining optimal conditions for cell growth and enzymatic activity. It is also employed in electrophoresis and chromatography techniques due to its minimal interference with biochemical reactions. Additionally, HEPES-Na is used in molecular biology applications, such as DNA and RNA hybridization, where consistent pH is critical for accurate results. Its low toxicity and compatibility with various biological systems further enhance its utility in laboratory settings.
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| Test Parameter | Specification |
|---|---|
| Purity (Titration) | 99.5-100.5 |
| UV Absorbance 260 nm (C = 1 M) | 0-0.4 |
| UV Absorbance 280nm (c=1m) | 0-0.2 |
| Water (Karl Fischer) | 0-3 |
| Appearance | White To Light Yellow Powder |
| Infrared Spectrum | Conforms To Structure |
| Solubility in Water | Colorless and Clear (500 mg/mL) |
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