Skimmin is primarily used in biochemical research as a fluorescent substrate or tracer due to its fluorescence under ultraviolet light. It is particularly suitable for assaying enzyme activity, especially β-glucosidase, which cleaves the glucose bond in skimmin to produce a highly fluorescent compound, enabling easy and accurate measurement of results.

Additionally, skimmin is employed in studies of the biological effects of plant extracts, particularly research on antioxidant and anti-inflammatory properties, where it shows potential in protecting cells from free radical damage.

In cosmetics and skincare products, it is utilized for its antioxidant properties, helping to protect the skin from environmental stressors and oxidative damage. It is also studied for potential anti-inflammatory effects, making it useful in formulations for sensitive or irritated skin. Found in some brightening serums to support skin clarity and even tone, and commonly included in herbal extracts for anti-aging products due to its natural origin and skin-soothing benefits.

In environmental applications, skimmin serves as a biological indicator (biomarker) to assess plant or soil health, especially in ecosystems affected by pollution or environmental stress.