Used as a stable isotopically labeled internal standard in mass spectrometry and nuclear magnetic resonance (NMR) studies to trace metabolic pathways and reaction mechanisms. Its carbon-13 label at the second position allows precise tracking of acetate incorporation in biochemical processes such as lipid synthesis, citric acid cycle activity, and acetylation reactions. Commonly applied in pharmaceutical research, metabolic profiling, and environmental studies where accurate quantification and pathway analysis are required. Also employed in labeled media for microbial fermentation studies to monitor substrate utilization and product formation with high sensitivity and specificity.