3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate

Ultra sensitive, used in ELISA

  • Product Code: 242955
  Alias:    TMB
  CAS:    54827-17-7
Molecular Weight: 240.34 g./mol Molecular Formula: C₁₆H₂₀N₂
EC Number: 259-364-6 MDL Number: MFCD00007748
Melting Point: 168-171 °C(lit.) Boiling Point: 368.6 °C at 760 mmHg
Density: Storage Condition: 2-8°C, dry, sealed
Product Description: Widely used in enzyme-linked immunosorbent assays (ELISA), this substrate delivers high sensitivity when detecting horseradish peroxidase (HRP) activity. It produces a stable blue color upon oxidation, allowing visual or spectrophotometric detection. The reaction is easily stopped with acid, shifting the color to yellow for optimized absorbance reading. Commonly applied in medical diagnostics, food safety testing, and research labs for detecting antigens and antibodies. Its low toxicity compared to similar substrates makes it suitable for routine lab use. Also employed in western blotting and other immunochemical techniques requiring clear, consistent signal development.
Sizes / Availability / Pricing:
Size Availability Price Quantity
100ml 10-20 days ฿7,800.00
+
-
500ml 10-20 days ฿23,300.00
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-
3, 3′,5 ,5′-Tetramethylbenzidine Liquid Substrate
Widely used in enzyme-linked immunosorbent assays (ELISA), this substrate delivers high sensitivity when detecting horseradish peroxidase (HRP) activity. It produces a stable blue color upon oxidation, allowing visual or spectrophotometric detection. The reaction is easily stopped with acid, shifting the color to yellow for optimized absorbance reading. Commonly applied in medical diagnostics, food safety testing, and research labs for detecting antigens and antibodies. Its low toxicity compared to similar substrates makes it suitable for routine lab use. Also employed in western blotting and other immunochemical techniques requiring clear, consistent signal development.
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