Polyamide powder (80-100 mesh) is a specialty adsorbent used in column chromatography for the separation and purification of polar organic compounds, such as flavonoids, alkaloids, phenols, amino acids, and peptides. It excels in normal-phase chromatography due to its strong hydrogen-bonding capabilities, providing high selectivity for compounds with hydroxyl, carbonyl, or amino groups. This reagent is essential in natural product isolation, pharmaceutical analysis, and synthetic organic chemistry.