Protopine hydrochloride is primarily used in research settings due to its potential pharmacological properties. It has been studied for its effects on the central nervous system, particularly in relation to its antispasmodic and analgesic properties. Researchers are exploring its potential in managing conditions like muscle spasms and pain. Additionally, it has shown promise in preliminary studies for its anti-inflammatory and antimicrobial activities, making it a candidate for further investigation in treating infections and inflammatory disorders. Its role in modulating neurotransmitter activity is also being examined, which could have implications for neurological and psychiatric conditions.