Pulsatilla saponin D is widely used in traditional medicine for its anti-inflammatory, antimicrobial, and pain-relieving properties. It is often incorporated into herbal remedies to treat conditions like respiratory infections, skin disorders, and digestive issues. In modern research, it has shown potential as an anticancer agent due to its ability to induce apoptosis and inhibit the growth of certain cancer cells. Additionally, it is studied for its immunomodulatory effects, which could help in developing treatments for autoimmune diseases. Its application in cosmetics is gaining attention for its skin-soothing, anti-aging, and antioxidant benefits. In agriculture, it is used as a biopesticide to control pests and plant diseases, enhancing crop efficiency.