UV-VIS Antioxidant Capacity (IC50 using ABTS)
- Product Code: 35448
The antioxidant activity of the test sample was measured with the ABTS (2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)) assay by Microplate Reader which is the most popular method to evaluate the ability of compounds to scavenge free radicals. The ABTS assay was adapted to measure the antioxidant activity of natural products, extracts, and compounds.
insert_drive_file Report of Previously Tested Sample
timeline Service Steps
| Step | Procedure | Expected Result |
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1
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Weigh 10... |
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A clear extract solution ready for antioxidant testing. |
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2
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Prepare Vitamin... |
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A series of standard solutions to establish a standard curve for antioxidant capacity and IC50 determination. |
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3
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Add 20... |
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sample |
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4
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Add 180... |
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sample and ABTS misture |
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5
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Mix the... |
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discoloration of reagent |
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6
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Each measurement... |
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% inhibition |
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7
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A standard... |
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standard curve |
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8
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IC50 is... |
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IC50 |
You will receive a report for the %inhibition , The value of IC 50 fields when we provide this service
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