Targocil is primarily used in the field of microbiology and pharmaceutical research as a potent inhibitor of bacterial cell wall synthesis. It specifically targets the late stages of peptidoglycan biosynthesis, making it a valuable tool for studying Gram-positive bacteria, particularly Staphylococcus aureus. Researchers utilize Targocil to investigate antibiotic resistance mechanisms and to develop novel therapeutic strategies against bacterial infections. Its application extends to screening for new antibacterial agents and understanding the molecular pathways involved in cell wall assembly. Additionally, Targocil serves as a reference compound in assays designed to evaluate the efficacy of potential antibacterial drugs.