Walrycin B is primarily utilized in the field of medical research due to its potent antibacterial properties. It is particularly effective against a range of Gram-positive bacteria, making it a valuable compound in the study of new antibiotic therapies. Researchers are exploring its potential in treating infections that are resistant to conventional antibiotics, thereby addressing the growing concern of antibiotic resistance. Additionally, Walrycin B is being investigated for its role in combating bacterial biofilms, which are often resistant to standard treatments. Its application extends to the development of novel pharmaceutical formulations aimed at enhancing the efficacy and delivery of antibacterial agents.