Rolitetracycline is primarily used as a broad-spectrum antibiotic in both human and veterinary medicine. It is effective against a wide range of bacterial infections, including respiratory, urinary tract, and skin infections. In veterinary applications, it is commonly administered to livestock to treat bacterial diseases and promote growth. The compound is often used in injectable forms for rapid action and better bioavailability. Additionally, it has been employed in research settings to study bacterial resistance mechanisms and antibiotic efficacy. Its use has declined in recent years due to the emergence of more advanced antibiotics and concerns over antibiotic resistance.