Amifloxacin (Win49375) is a fluoroquinolone antibiotic primarily used in veterinary medicine as a broad-spectrum antibacterial agent. It is effective against both Gram-negative and Gram-positive bacteria by inhibiting the enzymes DNA gyrase and topoisomerase IV, which are essential for bacterial DNA replication and cell division. In animals, particularly livestock and poultry, it is administered to treat respiratory and gastrointestinal infections, improving health and productivity. Its activity also aids in preventing and controlling bacterial diseases in farming operations. Additionally, amifloxacin is used in aquaculture to manage bacterial infections in fish, supporting the health and sustainability of aquatic populations. Proper dosage and administration are essential to minimize antibiotic resistance and ensure effective treatment.